Webb9 juni 2024 · 伯乐Bio-rad 732-6223TrisMicro Bio-Spin Columns with Bio-Gel P-30 in Tris Buffer缓冲液中P-30生物凝胶微生物旋转柱. Bio-Rad伯乐公司总部设在美国加里福尼亚州的Hercules,Bio-Rad在quanqiu拥有30多家全资子公司,服务于quanqiu超过85,000家生物技术公司、生物制药公司、科学研究机构和临床实验室。 WebbHIS-Select® Spin Columns are selective for recombinant proteins with His-tags and exhibit very low non-specific binding of other proteins. The selectivity can be modulated …
HIS-Select Spin Columns (H7787) - Technical Bulletin
http://www.bjnobleryder.com/nobleryder-Products-35418233/ WebbSpin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions. Procedure [ edit] The stages of the method are lyse, bind, wash, and elute. britt weyts
Technical Bulletin HIS-Select Spin Columns
WebbThermo Scientific Pierce Screw Cap Spin Columns are convenient tools for manipulating small volumes of affinity supports (20 to 400 L) for protein purification.Features of Screw Cap Spin Columns: Adaptable: inc Pierce™ 离心柱 - 螺旋盖 You need to enable JavaScript to run this app. Thermo Fisher Scientific Logo 登录 没有账户? 建立账号注 … Webb9 maj 2014 · Large scale preparation was carried out using 100 ml of induced cultures. Bacteria were pelleted by centrifugation and resuspended in CellLytic B reagent (Sigma). After centrifugation the soluble fraction of the lysate was used for protein purification using HisSelect™ Spin Columns (Sigma) according to manufacturer’s protocol. WebbBacterial lysate (1.0 mg total protein) was applied to a 0.2 mL bed volume of HisPur Cobalt Resin in a spin column. The resin was washed three times with 0.4 mL of wash buffer containing 10 mM imidazole. His-tagged proteins were eluted three times with 0.2 mL of elution buffer containing 150 mM imidazole. captured book