The gus gene fusion system

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Web10 Apr 2024 · The aphid feeding as well as aphid microbiota induced the vasculature expression of gus gene. An induction of gus expression which was under a control of pad4 promoter was evidenced at the site of aphid stylet on vasculature region (Fig. 5A.III). Likewise, the sag13 gene expression was induced from an aphid herbivory at RNA level … Web9 Aug 2016 · YFP fusion proteins with AtORM1 and AtORM2 were prepared by amplification of the AtORM1 and AtORM2 open reading frames using gene-specific primers (P16–P19; Supplemental Table S1). PCR products were cloned into the 35S-pFAST-eYFP vector using the Sac I and Kpn I restriction sites generating C-terminal YFP fusion constructs.

Histone Acetylation Accompanied with Promoter Sequences

Web6 Apr 2024 · A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene … Websimilar to the MsMADS1::GUS fusion constructs, except for the gene of interest. These constructs were named R079 and R079+. Final constructs in pCambia are shown in figure 12. ... transient gene expression system for intact leaves, Plant Science 122 : 101-108. Khoudi H, Laberge S, Ferullo JM, Bazin R, Darveau A, Castonguay Y, Allard G, Lemieux ... can you use tea tree oil on face

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Web1 day ago · The PbHMGR1 promoter sequence was inserted into the pCAMBIA1305.1 vector (TransGen Biotech, China) using EcoRI/NcoI sites in front of the β-glucuronidase (GUS) reporter gene. The CDS of PbHMGR1 was inserted into the pCAMBIA1305.1 vector harboring the Green Fluorescent Protein ( GFP ) gene in the place of GUS , promoted by the CaMV … Web1 May 1995 · Enhanced GUS gene expression in cereal/grass cell suspesions and immature embryos using the maize ubiquitin-based plasmid pAHC25. Plant Cell Reports 12 : … Web1 Dec 1995 · It is shown that following PAGE, both fluorometric and histochemical substrates can be used with equal efficiency for the detection of GUS activities as stained bands in gels following protein electrophoresis. Here we describe an as yet neglected application of GUS fusion proteins, the highly sensitive GUS activity staining of protein … can you use teflon tape on oxygen fittings

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GUS as a marker to track microbes SpringerLink

Webfrom AtPUB24 upstream of the GUS reporter gene. T2 and T3 plants from four independent lines were analyzed for glucuronidase (GUS) expression. ... (Invitrogen, Carlsbad, U.S.A.) in an Mx3000P QPCR System (Stratagene, California, U.S.A.). Reaction volume was 20µl and contained 10µl of the reaction mix, 0.5nmol of each primer and 2µl of cDNA ... Web28 Feb 2001 · A T-DNA-mediated translational fusion of AtTOR with the GUS reporter gene allows us to show that AtTOR is expressed in primary meristem, embryo, and endosperm, but not in differentiated cells. ... The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell ... can you use tea tree oil shampoo everydayWeb12 Apr 2024 · Blood biomarker-based methods to diagnose MI are the immediate solutions, and the cardiac biomarker troponin I (cTnI) was detected on an interdigitated electrode sensor. The limit of detection with aptamer-gold-cTnI-antibody was 100 aM and 1 fM with antibody-gold-cTnI-aptamer. Abstract Full text PDF References Request permissions … can you use tea tree shampoo daily

"WebAssaying chimeric genes in plants: The GUS gene fusion system Richard A. Jefferson Plant Molecular Biology Reporter 5 , 387–405 ( 1987) Cite this article 6593 Accesses 3077 … " - The gus gene fusion system

The gus gene fusion system

WebThe plant-pathogenic fungus Fusarium oxysporum was successfully transformed with the beta-D-glucuronidase gene from Escherichia coli (gusA) (GUS system) in combination … WebDoctor of biology with extensive experience as a senior scientist and a VP R&D in biotech companies. R&D expertise in molecular biology, protein engineering, genetic engineering. Inventor of eight patents; co-author of a professional book chapter and six articles. Experienced in research planning, leading scientific projects, patenting. Specializing …

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WebThe E. coli GUS ( -glucuronidase) gene is extensively used as a gene fusion marker for analysis of gene expression in transformed plants. The GUS reporter gene system has many advantages including the stability of the expressed E. coli Reagent CGUS enzyme and the low intrinsic activity of GUS in higher plants. The WebJefferson, R. A. (1987). Assaying chimeric genes in plants: The GUS gene fusion system. Plant Molecular Biology Reporter, 5(4), 387–405. doi:10.1007/bf02667740

WebThe GUS gene fusion system (Escherichia coli beta-D-glucuronidase gene), a useful tool in studies of root colonization by Fusarium oxysporum. Y Couteaudier, M J Daboussi, A … WebExpression of GUS gene was studied in transgenic plants under the infection of A. flavus. The analysis of AhOMT1 gene characterized by in silico assay, RNAseq, and qRT-PCR revealed minute expression in different organs and tissues with trace or no response to low temperature, drought, hormones, Ca2+, and bacterial stresses, but highly induced by A. …

The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology and microbiology. Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical … See more The purpose of this technique is to analyze the activity of a gene transcription promoter (in terms of expression of a so-called reporter gene under the regulatory control of that promoter) either in a quantitative manner, … See more There are different possible glucuronides that can be used as substrates for the β-glucuronidase, depending on the type of detection needed ( See more An organism is suitable for a GUS assay if it lacks naturally occurring β-glucuronidase activity or if the activity is very low (background activity). For this reason, the assay is not useful in most vertebrates and many molluscs. Since there is no detectable GUS … See more The GUS system is not the only available gene reporter system for the analysis of promoter activity. Other competing systems are based on e.g. luciferase, GFP, beta-galactosidase, chloramphenicol acetyltransferase (CAT), alkaline phosphatase. … See more The system was originally developed by Richard Anthony Jefferson during his Ph.D. at the University of Colorado at Boulder. He adapted the technique for the use with plants as he worked in the Plant Breeding Institute of Cambridge, between 1985 and 1987. Since then … See more The GUS assay does not require the presence of any cofactors or ions for function. Beta-glucuronidase can function through a wide … See more The GUS assay, as well as other reporter gene systems, can be used for other kinds of studies other than the classical promoter activity assay. Reporter systems have been used for the determination of the efficiency of gene delivery systems, the intracellular … See more Web1 Jul 1993 · The GUS gene fusion system (Escherichia coli ??-D-glucuronidase gene), a useful tool in studies of root colonization by Fusarium oxysporum July 1993 Applied and …

Web21 Jul 2024 · Jefferson, A.R. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Rep. 1987, 5, 387–405. [Google Scholar] Bradford, M.M. A rapid …

Web13 Apr 2024 · Approximately 2000 bp of CsSHBY upstream promoter sequences from the genomic DNA of CCMC were cloned into pCAMBIA 1304-GUS vector to obtain GUS fusion expression vector. The CDS ... system (AGILENT, USA). ... (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J … can you use tea tree shampoo on dogsWeb1 May 1994 · GUS activity under the control of the PEP protein promoter was observed later in infection and its maximal expression was less than 10% the level for p10 promoter-GUS constructs. ... the GUS gene fusion system. ... Hirsh D. 1986; β-glucuronidase from Escherichia coli as a gene-fusion marker. Proceedings of the National Academy of … can you use tea tree oil shampoo on dogsWebinto the genome of a reporter gene under the control of a mini- Functional GUS fusion frequencies ranged from about 2 to mal or no promoter can generate a transcriptional fusion with a 5% of the total number of transgenic lines. ... genes was used as a delivery system for the fusion constructs, and the resulting transgenic hairy roots were ... british birds eggs identification chartWeb1 May 2024 · An efficient biolistic transformation system of banana combined with a liquid medium selection system was developed during this study. An embryogenic cell suspension (ECS) of Musa acuminata cv. Baxi (AAA) was bombarded with a particle delivery system. After 7 days of restoring culture in liquid M2 medium, embryogenic cells were transferred … british birds finches picturesWeb11 Apr 2024 · The promoter of the CmSTM gene was constructed on PCAMBIASuper1304 to start the expression of the GUS gene on the vector, ... Then the fusion expression plasmid was transiently transformed into chrysanthemum protoplasts by the PEG transformation method. The reaction system was 300 μL protoplast cells, 30 μL plasmid, and PEG4000 … can you use tea tree oil on cold soresWebThe efficiency of vir gene influence and conditions of T-DNA delivery belong important parameters with developing an efficient Agrobacterium-mediated factory transformation system.Reliable selection regressions coupled with einen efficiency recycling method are important for acquisition stably transported install. british birds bookWebABSTRACT: To enhance the antifungal response of blackgram (Vigna mungo L.), transgenic plants were generated by transferring bacterial chitinase gene with a CaMV 35S promoter. … can you use teflon tape on gasoline lines